Preparation of gold Nanoparticles Using a Green Method with Papaya Leaf Extract and Characterization Using UV-Vis, FTIR and XRD
Keywords:
Gold nanoparticles, papayaleaf extract, UV-Vis, FTIR, XRDAbstract
Green synthesis of gold nanoparticles (AuNPs) has gained popularity as an economical and eco-friendly substitute for traditional chemical processes. Papaya leaf extract was used in this work as a natural reducing and stabilizing agent to create gold nanoparticles. When papaya leaf aqueous extract and chloroauric acid solution were combined under moderate reaction conditions, the synthesis of AuNPs was verified by a noticeable color shift. X-Ray Diffraction (XRD), ultraviolet–visible (UV–Vis) spectroscopy and Fourier transform infrared spectroscopy (FTIR) were used to analyze the produced gold nanoparticles. A distinctive surface plasmon resonance (SPR) absorption band at 520–550 nm was found by UV–Vis examination, demonstrating the stability and successful production of gold nanoparticles. The XRD patterns showed the strong crystallinity of the biosynthesized nanoparticles and verified the face-centered cubic (FCC) structure of gold, FTIR analysis confirmed the presence of phytochemicals from Carica papaya leaf extract as capping agents. The findings show that papaya leaf extract works well as a green agent for producing gold nanoparticles without the usage of hazardous substances.
References
. A. Bukhari, I. Ijaz, E. Gilani, A. Nazir, H. Zain, R. Saeed, S. S. Alarfaji, S. Hussain, R. Aftab, and Y. Naseer, “Green synthesis of metal and metal oxide nanoparticles using different plants’ parts for antimicrobial activity and anticancer activity: A review,” Coatings, vol. 11, no. 11, Art. no. 1374, 2021, doi: 10.3390/coatings11111374.
. P. V. Nagime and V. S. Chandak, “A comprehensive review of nanomaterials synthesis: Physical, chemical, and biological approaches and emerging challenges,” Biocatalysis and Agricultural Biotechnology, vol. 62, Art. no. 103458, 2024, doi: 10.1016/j.bcab.2024.103458.
. [ J. J. K. Ribeiro, P. S. da Silva Porto, R. D. Pereira, and E. P. Muniz, “Green synthesis of nanomaterials: Most cited papers and research trends,” Research, Society and Development, vol. 9, no. 1, 2020.
. S. Nimesh, R. Chandra, and N. Gupta, Advances in Nanomedicine for the Delivery of Therapeutic Nucleic Acids. Cambridge, U.K.: Woodhead Publishing, 2017.
. K. K. Bharadwaj et al., “Green synthesis of gold nanoparticles using plant extracts as beneficial prospect for cancer theranostics,” Molecules, vol. 26, no. 21, Art. no. 6389, 2021, doi: 10.3390/molecules26216389.
. L. Castillo-Henríquez et al., “Green synthesis of gold and silver nanoparticles from plant extracts and their possible applications as antimicrobial agents in the agricultural area,” Nanomaterials, vol. 10, no. 9, Art. no. 1763, 2020, doi: 10.3390/nano10091763.
. F. Al-Sarraj et al., “Green synthesis of chitosan-capped gold nanoparticles using Salvia officinalis extract: Biochemical characterization and antimicrobial and cytotoxic activities,” Molecules, vol. 28, no. 23, Art. no. 7762, 2023, doi: 10.3390/molecules28237762.
. P. B. Santhosh, J. Genova, and H. Chamati, “Green synthesis of gold nanoparticles: An eco-friendly approach,” Chemistry, vol. 4, no. 2, pp. 345–369, 2022, doi: 10.3390/chemistry4020026.
. P. Burns, P. Saengmanee, and U. Doung-Ngern, Papaya: The Versatile Tropical Fruit. Bangkok, Thailand: National Center for Genetic Engineering and Biotechnology (NSTDA), 2023, doi: 10.5772/intechopen.104624.
. R. Srivastava, N. Jaiswal, H. Kharkwal, N. K. Dubey, and R. Srivastava, “Against viral infections,” Viruses, vol. 17, no. 2, Art. no. 271, 2025, doi: 10.3390/v17020271.
. A. Sharma et al., “Carica papaya L. leaves: Deciphering its antioxidant bioactives, biological activities, innovative products, and safety aspects,” Oxidative Medicine and Cellular Longevity, vol. 2022, Art. no. 2451733, 2022, doi: 10.1155/2022/2451733.
. S. P. Singh et al., “Therapeutic application of Carica papaya leaf extract in the management of human diseases,” DARU Journal of Pharmaceutical Sciences, vol. 28, no. 2, pp. 735–744, 2020, doi: 10.1007/s40199-020-00348-7.
. R. Choudhary et al., “Nutritional, phytochemical, and antimicrobial properties of Carica papaya leaves: Implications for health benefits and food applications,” Foods, vol. 14, no. 2, Art. no. 154, 2025, doi: 10.3390/foods14020154.
. A. N. E. Hamed et al., “Chemical constituents from Carica papaya Linn. leaves as potential cytotoxic, EGFRwt and aromatase (CYP19A) inhibitors,” RSC Advances, vol. 12, no. 15, pp. 9154–9162, 2022, doi: 10.1039/d1ra07000b.
. D. Dimitrios et al., “Phytochemical screening of Psidium guajava and Carica papaya leaves aqueous extracts cultivated in Greece and their potential as health boosters,” Exploration of Foods and Foodomics, pp. 5–14, 2023, doi: 10.37349/eff.2023.00002.
. V. Amendola et al., “Surface plasmon resonance in gold nanoparticles: A review,” Journal of Physics: Condensed Matter, vol. 29, no. 20, Art. no. 203002, 2017.
. E. Boisselier and D. Astruc, “Gold nanoparticles in nanomedicine: Preparations, imaging, diagnostics, therapies and toxicity,” Chemical Society Reviews, vol. 38, no. 6, pp. 1759–1782, 2009.
. S. Sunkari et al., “Microwave-irradiated green synthesis of gold nanoparticles for catalytic and antibacterial activity,” Journal of Analytical Science and Technology, vol. 8, Art. no. 13, 2017.
. M. A. Alghuthaymi et al., “Green synthesis of gold nanoparticles using Polianthes tuberosa L. floral extract,” Plants, vol. 10, no. 11, Art. no. 2370, 2021, doi: 10.3390/plants10112370.
. T. A. Sathya et al., “Environmental profiling of gold nanoparticles by flavonoids fractionalization from Carica papaya leaf extract for photocatalytic degradation of organic contaminants and cytotoxic analysis,” Environmental Research, vol. 259, Art. no. 119445, 2024, doi: 10.1016/j.envres.2024.119445.
. M. Barasinski et al., “In situ tracking of nanoparticles during electrophoresis in hydrogels using a fiber-based UV–Vis system,” Nanomaterials, vol. 4, no. 1, Art. no. 3, 2025.
. S. Malik et al., “Cytotoxicity study of gold nanoparticle synthesis using Aloe vera, honey, and Gymnema sylvestre leaf extract,” ACS Omega, vol. 8, no. 7, pp. 6325–6336, 2023, doi: 10.1021/acsomega.2c06491.
. A. M. Pinilla-Torres, C. N. Sanchez-Dominguez, K. Basilio-Bernabe, P. Y. Carrion-Garcia, J. A. Roacho-Perez, E. N. Garza-Treviño, H. Gallardo-Blanco, and M. Sanchez-Dominguez, “Green synthesis of mesquite-gum-stabilized gold nanoparticles for biomedical applications: Physicochemical properties and biocompatibility assessment,” Polymers, vol. 15, no. 17, Art. no. 3533, 2023, doi: 10.3390/polym15173533.
. N. Wehbe et al., “Halodule uninervis extract facilitates the green synthesis of gold nanoparticles with anticancer activity,” Scientific Reports, vol. 15, no. 1, Art. no. 4286, 2025, doi: 10.1038/s41598-025-10822-z.
. C. Vázquez-Morón, F. Serrano-Marugán, M. Orzáez, B. Suay-García, and J. López-Martínez, “Plant-based extracts as reducing, capping, and stabilizing agents for the green synthesis of inorganic nanoparticles,” Resources, vol. 13, Art. no. 70, 2024, doi: 10.3390/resources13050070.
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Copyright (c) 2026 Adel M. Mlitan، Salima M. Farhat، Ali M. Alhalib
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