Infrared spectra and anti mitotic potential of Linum usitatissimum L gel water extract

Adel Mlitan

المؤلفون

Adel Mlitan

الكلمات المفتاحية:

IR properties، Linseed، Mitodepressant، Genotoxicity

الملخص

This study was conducted at Misurata City in Libya. Gel of linseed is primary a mixture of polysacharies which acid-catalyzed hydrolysis yield rhamonose fucose arabinose, xylose, glactose, galacturonic acide, and glucose. It used as an emulsifying agent for chocolate milk and functionally resembles arbic gum. In this study gel of linseed isolated from locally, Linseeds by hot water extract. Antimitotic action potential of extracted gel investigated using Allium cepa bioassay. Different concentration of gel (2.5%, 5%) were tested at different periods (2, 4, 6hours) on active growing root tip cells .Changes in the rate of cell division (mitotic index) was recorded. The result showed a significant decrease in mitotic index in treated cells in comparison with control. The result showed also that gel of linseed has more mitodepressant action than whole seed extract. IR of the extracted gel and whole seed extract were similar in band number and peaks. The data suggest that mitodepressnt effect of Linseed gel on rate of cell division may result of biologically active functional group of chemicals indicated by absorption spectra properties.

المراجع

. Abarzua, S., Szewczyk, M., Gailus, S., Richter, D. U., Ruth, W., Briese, V., and Piechulla, B. ( 2007); Effects of phytoestrogen extracts from Linum usitatissimum on the Jeg3 human trophoblast tumour cell line. Anticancer Res 27(4A):2053-2058.

. Bommareddy, A., Arasada, B. L., Mathees, D., and Dwivedi, C. (2006); Chemopreventive effects of dietary flaxseed on colon tumor development. J of Nutr Cancer 54(2): 216-222.

[[3]. Cui, W. G., Mazza, B. D Omah and Biliaderis C.G (1994); Processing optimization and physicochemical characteristics of flex seed polysaccharides gums. IN Philips,G. O., Williams P. A., Wedlock D. J (eds). Gums and Stabilizers for the food Industry 7. Oxford University press. NY.p.193-148.

. Bhartty, R and Cherdkiatgumchai, P (1990); Compositional analysis of laboratory prepared and commercial samples of linseed meal. J. AM. Oil Chem. Soc. 67:79-84.

. Fedeniuk, R and Costas, B. (1994); Composition and Physiochemical properties of linseed (Linum usitatissimum) Mucilage. J. Agric, Food Chem. 42: 240- 247.

. Al-Aubadi, M. and Al- Jobouri., A. H. (2013); Accessing the physiochemical and functional properties of flaxseed mucilage. J. of Iraqi of Agricultural Science. 44 (6):745-753.

. Grant, W.F.(1982); Chromosome aberration assays in Allium Mutation Research. 99:237-291).

. Antonise-Wiez, D.(1990); Analysis of the cell cycle in the root meristem of Allium cepa the influence of leda. Kirn. Hiostochem. Cytobiol. 26: 76-96.

. Basbülbül, G., Özmen, A., iyik, H and Şen, Ö. (2008); Anti mitotic and anti bacterial effects of the Primula veris L. flower extracts. Caryologia. 16 (1): 88-91.

. Peter, F and Tomaž, A (2013); Allium Chromosome Aberration Test for Evaluation Effect of Cleaning Municipal Water with Constructed Wetland (CW) in Sveti Tomaž, Slovenia. Firbas and Amon, J of Bioremed Biodeg.4-4.

. Hemakumar KH,,Sathisha A D ,Y B Basavaraju (2016); Synthesis and Anti- mitotic Activity Study Of New Tetralone Ester Intermediates of Anti cancer Agent Beta-Apopicropodophyllin. Indo American J of pharmaceutical Research. Vol 6 (5) 5505- 55011.

. Soliman, M. I.(2001); Genotoxicity testing of Neem plant (Azadirachta indica A. Juss) Using the Allium cepa chromosomes aberration assay. J of Biol. Sci., 1:1021-1027

. Nair V., Dal Z., Khan M., Henry P and Ciolino H.P (2011); Pomegranate Extract induces Cell Cycle Arrest and Alters Cellular Phenotype of Human Pancreatic Cancer Cells. Anticancer Res., 31: 2699-2704

. McCollum, G., Keng, P. C., States, J.C and McCabe, M.J., (2005); Arsenite delays progression through each cell cycle phase and induces apoptosis following G2/M arrest in U937 myeloid leukemia cells. J. Pharmaco. Exp. Ther., 313:877-887.

. Chin, C.F. and Yeong, F.M. (2010); Safe guarding entry into mitosis: the anaphase checkpoint. Mol. Cell Biol., 30:22-32

. SAKS, S., Upreti, R and Raman, D.(2017); GENOTOXICITY; Mechanism, Testing Guide lines and Methods. Glibl. J. of pharmaceutical Science.Vol 1(5): 1-6.

. El-Alfy, Z; Mahmoud F; Amany. A and Sally. E-(2016); Genotoxic Effect of Methotrexate on Bone Marrow Chromosomes and DNA of Male Albino Mice(Mus musculus). The Egyptian J. Hospital Medicine Vol.64,350-363.